Figure 11.

Accumulation of short and long CCUG repeats during transcription pulse does not alter the levels of ZNF9 mRNA. A: Total RNA was extracted at different time points after pulse of transcription as shown in Figure 5B, and was analyzed by real-time RT-PCR with ZNF9-specific primers (set 2, in Materials and Methods). ZNF9 signal was adjusted to GAPDH. X represents time (in hours) after Dox addition. Y represents the intensity of the ZNF9 signal as ratio to GAPDH signal. B: ZNF9 mRNA levels are not reduced in total RNA isolated from DM2 myoblasts. ZNF9 mRNA levels were measured by real-time RT-PCR in normal and DM2 myoblasts with two sets of ZNF9 specific primers and using GAPDH as a standard. C: ZNF9 mRNA levels are not reduced in the poly (A) fraction of RNA from DM2 myoblasts. Poly (A) mRNA was extracted from normal myoblast line and DM2 myoblast line. The levels of ZNF9 were quantified by real-time RT-PCR, with two sets of ZNF9 specific primers and GAPDH as control.