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. 2011 Nov 1;22(21):3986–3994. doi: 10.1091/mbc.E11-04-0379

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© 2011 Peng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Actin rearrangements are required for the spatial persistence of Rac activity in randomly migrating HL-60 cells. Rac activity was assayed by TIRF imaging of a fluorescent Rac-GTP–binding domain (Pak-PBD-Cerulean). (A) Control cells are polarized and maintain a persistent field of Rac activity at the leading edge. (B) In JLY-treated cells, the Rac activity field loses its persistent localization to the leading edge. White dotted line indicates the morphological leading edge. Images are representative of a minimum of five cells. Calibration bar indicates intensity (a.u.); scale bar: 10 μm. (C) Quantification of the spatial persistence of the Rac activity in control and JLY-treated HL-60 cells. Spatial persistence is measured by analyzing the vector between the center of mass of the cell and the center of mass of the Rac activity field over 11 min, then plotting the angle of those vectors over the fraction of time points in a rose plot.