TABLE 1:

Canavanine-resistant phenotypes of recycling mutants.

Strain	Score	Strain	Score	Strain	Score
Wild type	−	snx3Δ vps5Δ	++	snx41Δ snx3Δ	++++
snx4Δ	++	snx4Δ snx41Δ snx4Δ	++	snx4Δ snx3Δ	++++
snx41Δ	++	vps5Δ ere1Δ	++	snx4Δ vps35Δ	++++
snx42Δ	+	snx3Δ ere1Δ	++	snx4Δ ere1Δ	++++
snx3Δ	++	snx3Δ ere2Δ	++	snx41Δ ere1Δ	++++
vps5Δ	++	ere1Δ ere2Δ	++	snx41Δ ere2Δ	++++
vps17Δ	++			apl2Δ	−
vps26Δ	+			gga2Δ	−
vps29Δ	++			ent3Δ	−
vps35Δ	++			ent5Δ	−
ere1Δ	++
ere2Δ	++

The Ere1 and Ere2 proteins function in the retromer recycling pathway. Serial dilutions (fourfold) of wild-type and different mutant cells (as indicated) were grown on synthetic media plates with or without 2.0 μg/ml canavanine for 2 d at 26°C. The canavanine-resistant phenotype of each strain was scored based on the relative growth of serial dilutions on canavanine plates. Relative growth: no growth at 0.1 OD₆₀₀/ml was scored as −; growth at 0.1 OD₆₀₀/ml was scored as +; growth at 2.5 × 10⁻² OD₆₀₀/ml was scored as ++; growth at 1.56 × 10⁻³ OD₆₀₀/ml was scored as ++++.