FIGURE 2:

NNS-induced autophagy is important for cell growth upon switch from YPL to SL medium. (A) Growth curve of WT and atg mutant cells in YPL medium and after switch to SL medium. For growth measurements in YPL, cells of the indicated genotypes were cultured in YPL to log phase and then diluted to OD₆₀₀ 0.05–0.1. OD₆₀₀ was measured every 1–3 h after dilution and normalized against the first time point. For growth measurements after switch from YPL to SL medium, cells of the indicated genotypes were grown in YPL to log phase (OD₆₀₀ 0.1–0.15) and then switched to SL medium. OD₆₀₀ was measured every 24 h after medium switch and normalized against the first time point. (B) Growth curve of WT, Δatg5, and Δatg32 cells in YPL medium and after switch to SL medium. Growth measurements were performed as described in (A).