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. 2011 Nov 1;22(21):4171–4181. doi: 10.1091/mbc.E11-02-0163

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© 2011 Melvin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — ISWI controls RNA polymerase loading to the FIH gene. U2OS cells were depleted of ISWI using siRNA oligonucleotides. Chromatin immunoprecipitation was performed using RNA polymerase II (Pol II) (A), acetylated histone H3 (AcH3) (B), histone H3 (H3) (C), dimethyl-histone H3 (Lys36) (2MeK36) (D), and trimethyl-histone H3 (Lys36) (3MeK36) (E). Antibodies and enrichment measured by real-time quantitative PCR, normalizing to input and control siRNA. Bars represent the average of at least three independent biological replicates, and error bars represent the SE. P values were calculated using Student's t test (*p < 0.05; **p < 0.01; ***p < 0.001).