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. 2011 Nov 1;22(21):4192–4204. doi: 10.1091/mbc.E11-05-0467

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© 2011 Hickman et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Deleting MET4 causes slow growth, and this slow growth is spontaneously suppressed. (A) A met4Δ haploid grows poorly compared with MET4. A met4Δ/MET4 heterozygote (DBY12042) was sporulated, and the resulting tetrads were dissected on YPD plates. To show that the growth defect was not a simply a germination defect and persisted over many divisions, the spore colonies were restreaked onto fresh YPD plates and grown for 2 d (bottom). A MET4 wild type (FY4) is used as a control. Restreaking multiple times had the same effect. (B) The genomic locus surrounding the MET4 ORF (denoted by a box), showing that expression of the essential POL1 gene may be affected by deleting the MET4 ORF. (C) The met4Δ growth defect can be rescued by a plasmid containing MET4. A met4Δ/MET4 heterozygote containing either a MET4 plasmid (DBY12210, right) or a control plasmid (DBY12211, left) was sporulated, and the resulting tetrads were dissected on YPD plates. (D) When met4Δ haploid cells (DBY12213) are grown overnight in liquid rich medium, fast-growing cells arise in the population. This was repeated with met4Δ cells from ∼30 independent tetrads generated by sporulation of a met4Δ/MET4 heterozygote, with very similar results.