Figure 4.

Increased TIMP-2 and Ala+TIMP-2 levels reduce intratumoral microvascular density (MVD). A: Tumor angiogenesis was measured by immunohistochemical staining of blood vessels with an anti-mouse CD31 antibody in tumor sections from A549 xenografts. Representative photographs shown are from 20 high-power fields taken at ×200 magnification. Red arrows indicate examples of CD31-positive blood vessels. B: The graph shows the average number of CD31-positive vessels from 20 high-power fields (×400) per group (EV, T2, Ala+T2). Error bars indicate the mean MVD from the measurement of at least 10 animals per group. ***P < 0.0001 as compared with EV control. Significant difference also is observed between A549 T2 and Ala+T2 (***P = 0.0001).