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. 2011 Nov;179(5):2589–2600. doi: 10.1016/j.ajpath.2011.07.035

Figure 6.

Figure 6

FAK and AKT protein levels and activation are decreased in A549 tumors by immunoblotting and immunoperoxidase staining of A549 xenograft tumor cells. A: FAK Western Blotting in NOD-SCID derived A549 xenograft tumor cell lysates. Total FAK protein levels are reduced in A549 T2 and Ala+T2 tumors. Similarly, the phosphorylation of FAK Y397 is significantly decreased in both A549 T2 and Ala+T2 tumors compared with the EV control. Relative band intensities are shown below each blot. GAPDH is the loading control. B and C: Total FAK (B, left panels) and phospho Y397 FAK (B, right panels) and C: total AKT1 (C, left panels) and phospho AKT Ser473 (C, right panels) by IHC showing a decrease in their levels in both A549 T2 and Ala+T2 compared with EV control. The reduced levels of total FAK and AKT are shown predominantly in A549 T2, whereas, pFAK and pAKT are diminished in both T2 and Ala+T2 tumors. Magnification, ×200.