Table 2.
Distribution of lacZ+ Nuclei in GRMD Dog Muscles after MuStem Cell Delivery
| Dog number | Muscle | Number of lacZ+ nuclei | Tissue localization of lacZ+ nuclei |
||
|---|---|---|---|---|---|
| Subbasal position | Centronuclear position | Interstitial tissue | |||
| 8 | Gluteus superficialis | 3205 | 2445 (76.3%) | 320 (10.0%) | 440 (13.7%) |
| Triceps brachii | 2999 | 1865 (62.2%) | 693 (23.1%) | 441 (14.7%) | |
| Semitendinosus | 2540 | 1952 (76.9%) | 351 (13.8%) | 237 (9.3%) | |
| Total | 8744 | 6262 | 1364 | 1118 | |
| Percentage | 71.6% | 15.6% | 12.8% | ||
| 95% confidence interval | 70.7–72.6 | 14.9–16.4 | 12.1–13.5 | ||
| 9 | Triceps brachii | 549 | 510 (92.9%) | 38 (6.9%) | 1 (0.2%) |
| 10 | Triceps brachii | 641 | 598 (93.3%) | 42 (6.5%) | 1 (0.2%) |
| 11 | Triceps brachii | 536 | 503 (93.8%) | 32 (6.0%) | 1 (0.2%) |
| Total | 1726 | 1611 | 112 | 3 | |
| Percentage | 93.3% | 6.5% | 0.17% | ||
| 95% confidence interval | 92.2–94.5 | 5.3–7.7 | 0–0.4 | ||
| Dog number | Muscle | Number of lacZ+ nuclei | Tissue localization of lacZ+ nuclei |
||
|---|---|---|---|---|---|
| Below plasma membrane | Above basal membrane | Between both membrane | |||
| 12 | Biceps femoris | 326 | 238 (73.0%) | 35 (10.7%) | 53 (16.3%) |
| 13 | Biceps femoris | 314 | 217 (69.1%) | 43 (13.7%) | 54 (17.2%) |
| Total | 640 | 455 | 78 | 107 | |
| Percentage | 71.1% | 12.2% | 16.7% | ||
| 95% confidence interval | 67.6–74.6 | 9.7–14.7 | 13.8–19.6 | ||
| Dog number | Muscle | Number of lacZ+ nuclei | Tissue localization of lacZ+ nuclei |
||
|---|---|---|---|---|---|
| Subbasal position | Centronuclear position | Interstitial tissue | |||
| 14 | Semitendinosus | 127 | 100 (78.7%) | 0 (0%) | 27 (21.3%) |
| Gracilis | 239 | 191 (79.9%) | 0 (0%) | 48 (20.1%) | |
| 15 | Semitendinosus | 104 | 67 (64.4%) | 14 (13.5%) | 23 (22.1%) |
| Gracilis | 251 | 249 (99.2%) | 0 (0%) | 2 (0.8%) | |
| Total | 721 | 607 | 14 | 100 | |
| Percentage | 84.2% | 1.9% | 13.9% | ||
| 95% confidence interval | 81.5–86.9 | 0.9–2.9 | 11.4–16.4 | ||
Tissue localization of lacZ+ nuclei was determined on several skeletal muscles of eight dogs, 4 weeks after MuStem cell injection. Six dogs received intramuscular injection (#8 to #13), whereas two others received intra-arterial delivery (#14 and #15).