Figure 3.

Egr-1 staining in intra-acinar vessels in flow-associated PAH. A: Percentage of intra-acinar vessels staining positive for Egr-1 per experimental group. B: Localization of Egr-1 staining in intra-acinar vessels per experimental group. C: Typical examples (original magnification, ×400) of Egr-1 staining (arrows) in intra-acinar vessels per experimental group. Note that Egr-1 staining is located in endothelial cells 1 day after flow addition (T8). During disease development, Egr-1 staining is seen in the intima and media layer with strong Egr-1 staining in neointimal lesions. Data are presented as mean ± SEM. *P < 0.05 versus controls. CON, pooled sham groups. Scale bar = 50 μm.