Figure 3.

Leukocyte recruitment in trauma-induced inflammation after protein C (PC) treatment. Leukocyte adhesion (per mm² of surface area) and rolling flux fraction (RFF) (percentage) after trauma-induced inflammation were observed in mice treated with 100 U/kg PC at different time points before microscopic observation and in control mice, which received isotonic human serum albumin (8 mg/mL) for 3 hours. First, the number of adherent leukocytes (A) RFF (B) in surgically prepared cremaster muscle venules were analyzed under baseline conditions. Next, the additional increase of leukocyte adhesion over baseline (per mm² of surface area) is shown 3 minutes after systemic administration of 600 ng of chemokine (C-X-C motif) ligand 1 for the respective PC treatment intervals and control condition (C). Leukocyte transmigration (D) was observed in Giemsa-stained cremaster muscle whole mounts obtained after the respective intravital microscopic experiments. All values are presented as mean ± SEM from three or more mice per group. Significant differences (*P < 0.05) to control are indicated.