Figure 7.

Tumor necrosis factor-α (TNF-α)-stimulated intercellular adhesion molecule 1 (ICAM-1) expression on endothelial cells, β₂-integrin, and CXCR2 expression on neutrophils after PC treatment. ICAM-1 expression of cultured endothelial cells was measured after 4 hours of TNF-α stimulation (25 ng/mL) with or without prior PC pre-incubation (5 U/10⁶ cells) for 2 hours (A). Macrophage 1 antigen (Mac-1) and lyphocyte function-associated antigen 1 (LFA-1) blocking monoclonal antibody (mAb) were systemically injected into mice treated with 100 U/kg PC for 3 hours and a secondary antibody was used to detect Mac-1 and LFA-1 expression on peripheral blood neutrophils compared to control mice (B). Surface expression of CXCR2 on bone marrow neutrophils after pre-incubation with protein C (PC) (1 U/10⁶ cells for 3 hours) was compared to control (C). Representative histograms are shown from three separate experiments.