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. 1999 Apr;119(4):1557–1566. doi: 10.1104/pp.119.4.1557

Figure 3.

Figure 3

Accumulation of the PSII proteins. A, The equivalent of 2.5 μg of chlorophyll from each sample was analyzed by immunoblotting using antibodies directed against the D2 protein. B, The equivalent of 2 μg of chlorophyll from each sample was analyzed by immunoblotting using antibodies directed against the D1 or PsaA proteins. C, Thirty micrograms of protein extract from each sample was analyzed by immunoblotting using antibodies directed against the D1 protein. Dilutions of the wild type (WT) were done in FuD7 extracts to keep total protein concentration constant. D, The equivalent of 1.5 μg of chlorophyll from each sample was analyzed by 12.5% LDS-PAGE at 4°C. Immunoblotting was performed with antibodies directed against the D1 and D2 proteins.