Table II.
Growth properties of the transformants containing the psbD-aadA mutant genes
| Medium | Light | Spectinomycin | 61 (WT)a | 62 (T2D) | 63 (T2G) | 64 (T2E) | 65 (T2T) | nac2-26 | T2D | WT | psa CΔSpcs b |
|---|---|---|---|---|---|---|---|---|---|---|---|
| μE m−2 s−1 | μg mL−1 | ||||||||||
| TAP | 6 | 500 | −c | − | − | − | − | − | + | − | − |
| 6 | 300 | − | +d | − | − | − | − | + | − | − | |
| 6 | 200 | − | + | − | − | − | − | + | − | − | |
| 6 | 100 | + | + | − | − | − | − | + | − | − | |
| 6 | 50 | + | + | + | + | + | − | + | − | − | |
| 6 | 0 | + | + | + | + | + | + | + | + | + | |
| HSM | 60 | 0 | + | + | + | + | + | − | − | + | − |
a
WT, Wild type.
b
The psaCΔSpcs strain lacks psaC (Fischer et al., 1996) and was used as the recipient strain for transformation with the chimeric psbD-aadA genes.
c
−, No growth.
d
+, Growth.