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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Pharmacol Toxicol Methods. 2011 Jun 30;64(3):233–237. doi: 10.1016/j.vascn.2011.06.001

Figure 5.

Figure 5

Stacked film exposure improves background and signal in western blots.

Two ovarian cancer cell lines, A2780/CP70 and A2780/wt, were treated with different concentrations of kaempferol for 24 hours and harvested for SDS-PAGE and western blot with two different primary antibodies (Bax and Bad) and the same Goat-Anti-Mouse-Poly-HRP antibody. Blots were incubated with SuperSignal West Pico Chemiluminescent Substrate and exposed to two stacked films for 90 minutes. A. The 2nd film reduces background shown on the 1st film. B. Signal fold-changes were larger at film 2 because the strong signals may have reached saturation at film 1.