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. Author manuscript; available in PMC: 2012 Jul 26.
Published in final edited form as: Oncogene. 2011 Jun 27;31(4):494–506. doi: 10.1038/onc.2011.251

Figure 5. CALM2091AF10 localizes to the nucleus and does not influence Kit internalization in a myeloid line derived from CALM2091AF10+ AML cells.

Figure 5

(A) Protein expression of CALM2091AF10, detected using an anti-FLAG antibody, 48 hours after nucleofection with siRNA specific for human AF10 (hAF10) or non-targeting (NT) control siRNA in mouse CA2091-CL1 cells, an IL-3 dependent line derived from CALM-AF10+ AML mouse cells. (B) CA2091-CL1 cells were transfected with siRNAs: NT-non-targeting; hAF10-specific for CALM-AF10. Cells were treated with 200ng/ml SCF for times indicated, and KIT receptor remaining on the cell surface was measured. Fetal liver cells are shown as a positive control for KIT internalization. Knock-down of CALM-AF10 expression (hAF10 siRNA) does not increase KIT internalization. (C) Localization of CALM2091AF10, expressed in CA2091-CL1 cells, during interphase (1) and metaphase (2,3). Original magnification, 787.5X.