Epileptiform activity recurs after a single course of rapamycin treatment. Baseline EEG recordings were taken from NS-Pten conditional knockout mice at 4 weeks of age, before receiving a single course of rapamycin treatment during postnatal weeks 4–5. As compared to baseline, epileptiform activity was significantly reduced at weeks 6 and 9, but not at 10 weeks of age (Repeated Measures ANOVA, n=11), indicating a recurrence of epileptiform activity within 4 weeks after rapamycin withdrawal. *p<0.05.