Intermittent rapamycin treatment increases survival in NS-Pten conditional knockout mice. During postnatal weeks 4–5, NS-Pten knockouts were treated with vehicle solution (Vehicle, n=28, black trace), rapamycin (Rapa x1, n=20, blue), or left untreated (Naïve, n=13, grey). Additional knockout mice were treated with three courses of rapamycin during weeks 4–5, 10–11, and 16–17 (Rapa x3, n=16, red). Kaplan-Meier Logrank survival plot and analysis reveals Rapa x3 animals to live significantly longer than all other treatment groups (p=0.001), with a median age of 22 weeks as compared to 14 weeks or less in the vehicle and Rapa x1 groups.