FIG. 2.

The brain matrix coating (b, d, f) is able to support neurons derived from induced pluripotent stem cells. Highly branched neurons were identified on the brain matrix, which were not seen in the Matrigel coated wells (a, c, e). Cells were stained (c, d) using β-III-tubulin, a neuronal marker (green), and GABA-ergic neurons were identified through staining for GABA (red). Arrows identify highly branched neurons on the brain matrix coating. eGFP-transfected cells (e, f) were assessed for primary and secondary dendritic branching at day 8. Quantification of primary dendrites and secondary dendrites indicate statistically significant dendritic formation on the brain matrix coatings when compared with Matrigel coatings (g). Scale bar at 100 μm. **p<0.01.