FIG. 4.

Synapsin levels increased in both the Matrigel coated (a, c) and brain matrix coated (b, d) wells over time, where synapsin (red) is costained with β-III-tubulin, a general neuronal marker (green). One-week cultures (a, b) demonstrate less synapsin than at 2 weeks (c, d). Scale bar at 100 μm. Quantification of synapsin expression as area per field of view is demonstrated (e), and while there is no significance between the coatings, there is a trend of increased synapsin expression on the brain matrix at 2 weeks.