FIG. 2.

Characterization of PGS-PCL scaffolds in terms of their microstructure (A) and their ability to support MSC culture (B, C). (A) Field emission scanning electron microscope micrograph of a PGS-PCL scaffold. (B) Representative live/dead staining of MSCs after 3 days of preculture in the normal media. Live cells (green) and dead cells (red) were stained by Syto 13 and propidium iodide, respectively. The top panel is a top view of the confocal image overlapped with the phase contrast image. The bottom panel shows the side view of the constructs with 50 μm sampling depth. (C) Representative F-actin/vinculin costaining of MSCs after 3 days of preculture in the normal media. F-actin filaments were stained red by TRITC-phalloidin (indicated by the solid arrow), focal adhesion sites were stained green by anti-vinculin and FITC-labeled secondary antibody (indicated by the dotted arrow), and cell nuclei were counterstained blue by Draq 5. Color images available online at www.liebertonline.com/tea