FIG. 3.

Effects of CTGF on cell morphology (A, B) and scaffold cellularity (C). (A, B) MSCs were cultured in PGS-PCL scaffolds for 21 days in the normal (A) and conditioned (B) media and F-actin (red) and nuclei (blue) were stained by Alexa-568 phalloidin and DAPI, respectively. Images were obtained by merging confocal z-stack slices over 0–30 μm sample depth. (C) Cell proliferation as assessed by picogreen DNA quantification. DNA content was normalized to the dry mesh weight. ^#Significant difference (p<0.05) relative to the baseline level on day 0; **significant difference (p<0.05) between normal and conditioned culture. Color images available online at www.liebertonline.com/tea