Table 1.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | GenBank no. | Product size (bp) |
---|---|---|---|---|
COL I39 | AACAAATAAGCCATCACGCCT | TGAAACAGACTGGGCCAATGTC | NM_000089 | 101 |
ELA60 | AAAGCAGCAGCAAAGTTCGG | ACCTGGGAC AACTGGAATCC | NM_001081755 | 274 |
DEC61 | GATGCAGCTAGC CTGAAAGG | TCACACCCGAATAAGAAGCC | NM_133503 | 274 |
TIMP1 | TTTCTTGGTTCCCCAGAATG | CAGAGCTGCAGAGCAACAAG | NG_012533 | 99 |
HAS362 | TGTGCAGTGTATTAGTGGGCCCTT | TTGGAGCGCGTATACTTAGTT | NM_005329 | 177 |
CD4463 | TGCCGCTTTGCAGGTGTAT | GGCCTCCGTCCGAGAGA | NM_001001392 | 70 |
FSP-164 | AGCTTCTTGGGGAAAAGGAC | CCCCAACCACAT CAGAGG | NM_019554 | 200 |
aP265 | TCAGTGTGAATGGGGATGTG | GTGGAAGTGACGCCTTTCAT | NM_001442 | 250 |
ALP6 | TGGAGCTTCAGAAGCTCAACACCA | ATCTCGTTGTCTGAGTACCAGTCC | NM_000478 | 454 |
AGG66 | TCGAGGACAGCGAGGCC | TCGAGGGTGTAGCGTGTAGAGA | NM_013227 | 85 |
p5367 | TGCGTGTGGAGTATTTGGATG | GTGTGATGATGGTGAGGATGG | NM_000546 | 168 |
GAPDH68 | GAAATCCCATCACCATCTTCCAGG | GAGCCCCAGCCTTCTCCATG | NM_002046 | 120 |
Primer sequences for TIMP1 was designed based on gene sequence obtained from GenBank (NG_012533) using Primer 3 software (http://frodo.wi.mit.edu/primer3/). All other primer sequences were based on published records and the respective GenBank numbers are included in the table.