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Spiking recovery in human seruma
| Peptide concentration, ng/mL | Peptide serum + peptide concentration, ng/mL | Peptide recovered, ng/mL | Recovery, % |
|---|---|---|---|
| 31.1 | 145.3 | 31.1 | 103.5 |
| 13.4 | 135.3 | 13.3 | 110.4 |
| 7.6 | 125.7 | 7.6 | 107.6 |
| 4.7 | 121.6 | 4.7 | 106.7 |
| 0 | 109.2 | 0 | 100 |
aPeptide sample = native type IV collagen cleaved by matrix metalloproteinase 9 and pepsin. Recovery percentages are peptide concentrations compared to peptide measured in serum after subtracting the serum background.
