Table 1.
Oxidation currents for the different geometries
| Geometry | ΔImax [nA] direct detection of 4AP | ΔImax [nA] direct detection of pAPP | ΔImax [nA] enzymatic reaction | KM [mM] |
|---|---|---|---|---|
| S0 | 18.4 | 2.1 | 16.3 | 0.16 |
| S1 | 14.4 | 2.1 | 14.0 | 0.12 |
| S2 | 21.8 | - | - | - |
| I1 | 14.8 | - | 9.8 | 0.29 |
| I2 | 4.1 | - | 3.5 | 0.15 |
Maximum oxidation currents are recorded for different geometries S0, S1, S2, I1, I2 as recorded in phosphate buffer with pH = 7.8. Data are shown for detection of 4AP (cfg. Figure 4), pAPP (cfg. Additional File 1), and detection of 4AP after enzymatic degradation of pAPP with ALP (cfg. Figure 5). In the case of the enzymatic reaction also the Michaelis-Menten constant KM is given.