Figure 3.
Immune responses in mice vaccinated with GagCAT VLPs. IFN-γ (A) and IL-2 (B) ELISPOT responses of groups of BALB/c mice to Gag CD8 and Gag CD4 peptides on day 12 after vaccination with GagCAT VLPs (VLP) or heated GagCAT VLPs (hVLP) or pTHGagC and on day 40 for mice primed with pTHgagC then boosted on day 28 with VLPs or hVLPs. Splenocytes prepared from spleens combined from five mice per group were used in IFN-γ or IL-2 ELISPOT assays with the indicated Gag peptides. Bars are the mean number of spots of triplicate reactions for 106 splenocytes with indicated standard deviation of the mean. Data is that from one of three replicate experiments.