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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Plant J. 2011 Aug 30;68(4):633–645. doi: 10.1111/j.1365-313X.2011.04714.x

Figure 1. Schematic diagrams of the inducible RNAi construct and genomic structure of the PDS gene and phenotype of induced PDS silencing.

Figure 1

(A) A schematic diagram showing structural features of the inducible pX7-RNAi construct (see Guo et al. 2003 for detail), a chemical-inducible Cre/loxP (CLX) recombination system (Zuo and Chua 2000), to trigger the expression of an intron-containing inverted-repeat RNAi (IR). G10-90, a strong, synthetic, constitutive promoter._exo-Pdsi (exo-IR silencer): the reconstituted transcription unit derived from Cre/loxP-mediated DNA recombination after inducer treatment. endo-PDS (target): Genomic structure of the endogenous PDS gene. UTR, untranslated region of mRNA. Green boxes are exons of PDS separated by introns. Pds, fragment corresponding to the first 4 exons of PDS used for IR construction and probe for hybridization. (B) Phenotype of inducible PDSi at the post-germination stage. One-week-old seedlings were treated with inducer for one week and picture was taken at 10 days after removing inducer.