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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Pediatr Blood Cancer. 2011 Jul 26;58(5):722–728. doi: 10.1002/pbc.23282

Figure 3.

Figure 3

Extracted ion chromatograms (left) and amino acid sequence-specific MS/MS spectra (right) for targeted cLC/MS/MS of tryptic digests of SDS-PAGE gel slices at ~ 50 kDa revealed up-regulation of NF45 NF45 128–141 in 4 of 4 cESTs (0 of 4 DYSs) and confirmed up-regulation of FKBP4 190–206 in 4 of 4 DYSs (0 of 4 cESTs). Narrow cLC peaks with high signal-to-noise ratio in the extracted ion chromatograms for these peptides and protein database searching of their MS/MS spectra confirmed these results.