A. Growth curve of V. cholerae in RB medium with or without fatty acid supplementation (decanoate and/or oleate). The growth curve was recorded automatically with a BioScreen C instrument in which each time point was measured in five parallel wells. A representative result of three independent trails is shown.
B. Induction of V. cholerae plsB expression by oleate.
The LacZ (β-gal) data are expressed as the mean ± standard deviation and are from more than nine independent experiments. Strain FYJ176 (V. cholerae carrying pTL61T-PplsB_vc, a plasmid encoding a plsB-lacZ transcriptional fusion) was assayed.