Table 1.
Bacteria and plasmids in this study
|
E. coli strain or plasmid |
Relevant characteristics | Sources/References |
|---|---|---|
| Strains | ||
| MG1655 | Wild type stain of E. coli K-12 | Lab stock |
| JW1176-1 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) ΔfadR776::kan, rph-1 Δ(rhaD-rhaB)568, hsdR514 | CGSCa, (Baba et al., 2006) |
| Topo10 | ΔlacX74, cloning strain | Invitrogen |
| DH5α | Cloning strain | Lab stock |
| BL21(DE3) | E. coli protein expression strain | Lab stock |
| MFH8 | UB1005, fadR::Tn10 | (Henry & Cronan, 1992, Feng & Cronan, 2010, Feng & Cronan, 2011) |
| BB26-36 |
plsB26 plsX50 fadL701 glpR2(glpc) glpD3 glpK14(fbR) Encodes a defective G3Pacyltransferase, G3P auxotroph |
(Cronan & Bell, 1974) |
| MC4100 | araD139, Δ(argF-lac)169 | (Feng & Cronan, 2009b) |
| DH5α (λ-pir) | Δlac host for pAH125 and its derivatives | (Feng & Cronan, 2009a) |
| FYJ1 | DH5α (λ-pir) carrying pAH125 | (Feng & Cronan, 2009a) |
| FYJ57 | JW1176-1, ΔfadR | (Feng & Cronan, 2010, Feng & Cronan, 2011) |
| FYJ135 | MC4100 with a plsB_vc-lacZ fusion integrated into the chromosomal attλ site | This work |
| FYJ136 | plsB_vc-lacZ fusion, ΔfadR::Tn10 | P1vir(MFH8)×FYJ135 |
| FYJ158 | DH5α (λ-pir) carrying pAH125-PfadD_ec | This work |
| FYJ159 | MC4100 with a fadD_ec-lacZ fusion integrated into the chromosomal attλ site | This work |
| FYJ161 | FYJ57, ΔfadR, fadD-lacZ transcriptional fusion | P1vir(FYJ159)×FYJ57 |
| FYJ168 | Topo 10 carrying pTL61T-PplsB_vc | This work |
| FYJ176 | Vibrio cholerae carrying pTL61T-PplsB_vc | This work |
| FYJ187 | MC4100 pINT-ts | This work |
| Plasmids | ||
| pCR2.1-TOPO | Topo-cloning vector, AmpR, KanR, | Invitrogen |
| pET28a | T7 promoter-driven expression vector, KanR | Novagen |
| pAH125 | A promoter-less lacZ reporter plasmid used in E. coli, KanR | (Feng & Cronan, 2009a, Haldimann & Wanner, 2001) |
| pCR-plsBvc | pCR2.1 carrying V. cholerae plsB gene, AmpR, KanR | This work |
| pAH-PplsBvc | pAH125 carrying the promoter region of V. cholerae plsB gene, KanR | This work |
| pINT-ts | Temperature sensitive plasmid expressing phage λ Int. | (Haldimann & Wanner, 2001) |
| pTL61T | A promoter-less lacZ fusion plasmid used for V. cholerae | (Bellair & Withey, 2008, Linn & St Pierre, 1990, Withey & Dirita, 2005) |
| pTL61T-PplsBvc | pTL61T carrying the promoter region of the V. cholerae plsB gene. | This work |
| pET28-fadRec | Recombinant plasmid carrying the E. coli fadR gene, KanR | (Feng & Cronan, 2009b, Feng & Cronan, 2009a, Iram & Cronan, 2005, Feng & Cronan, 2010) |
| pET16-fadRvc | Recombinant plasmid carrying the V. cholerae fadR gene | Lab stock |
a
CGSC denotes Coli Genetic Stock Center, Yale University.