Table 2.
Primers used in this study
| Primers | Primer sequences |
|---|---|
| plsBvc-Fa | 5'-CG GGATCC CTG GAT TGC GTC CTC CAT AAT G-3’ |
| plsBvc-Ra | 5'-CCG CTCGAG GTT TGG TAA TCC CAG CTT AAG GC-3’ |
| plsBvc_FadR_BS-Fb | 5’-TTA AAT TAA AAG GTT TGA CCA GTT TCT GGT ATT CTT GGC-3’ |
| plsBvc_FadR_BS-Rb | 5'-GCC AAG AAT ACC AGA AAC TGG TCA AAC CTT TTA ATT TAA-3' |
| fadDec_FadR_F2 | 5’-GTA ATT ATC AAG CTG GTA TGA TGA GTT AAT ATT ATG-3’ |
| fadDec_FadR_R2 | 5’-CAT AAT ATT AAC TCA TCA TAC CAG CTT GAT AAT TAC-3’ |
| plsBvc_P-F | 5'-GTC CTC CAT AAT GGC TTC AAA G-3' |
| plsBvc_P-R | 5'-GTT CAA AGC CTC AAG ATT CTT G-3' |
| PplsBvc-F1a | 5'-CCG GTCGAC CAG TTG ACT GTG AGT ATA TCC AG-3' |
| PplsBvc-R1a | 5'-AACC GAATTC AGA GTT CAA AGC CTC AAG ATT C-3' |
| PplsBvc-F2a | 5'-CCG CTCGAGCAG TTG ACT GTG AGT ATA TCC AG-3' |
| PplsBvc-R2a | 5'-CG GGATCC AGA GTT CAA AGC CTC AAG ATT C-3' |
| plsBvc-Nested (212–235) | 5'-CAT TCA GCA TCA AAG GCT CTA AAG-3' |
| plsBvc-GSP (212–235) | 5'-GAA TCA CCT GCA CAT CCA ACT C-3' |
| RLM-RACE Adaptor | 5’-GCU GAU GGC GAU GAA UGA ACA CUG CGU UUG CUG GCU UUG AUG AAA-3’ |
| RLM-RACE Outer | 5’-GCT GAT GGC GAT GAA TGA ACA CTG-3’ |
| RLM-RACE Inner | 5’-CGC GGA TCC GAA CAC TGC GTT TGC TGG CTT TGA TGA AA-3’ |
| fadD-promoter-Fa | 5'-CCG GTCGAC GTT GCG GTA CAA AAC CAG CA-3' |
| fadD-promoter-Ra | 5'-AACC GAATTC CTC TAA AAT GCG TGT TCG TCG-3' |
| fadD-check2 | 5'-GTC TGA CGA CTG ACT TAA CGC-3' |
| lacZ-R | 5’-GAC CAT GAT TAC GGA TTC ACT G-3’ |
| 16Svc-F | 5’-GGA AAC GAT GGC TAA TAC CGC A-3’ |
| 16Svc-R | 5’-AGT GTG GCT GAT CAT CCT CTC A-3’ |
a
The underlined italic sequences are the introduced restriction sites.
b
The bold letters are the predicted core FadR binding palindromes.