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. Author manuscript; available in PMC: 2011 Oct 30.
Published in final edited form as: Am J Hypertens. 2011 Jun 30;24(10):1149–1155. doi: 10.1038/ajh.2011.114

Figure 1.

Figure 1

Effect of IRS-1 siRNA on Akt phosphorylation and protein synthesis. (a) VSMCs were transfected with IRS-1 siRNA or scrambled siRNA for 48 h and then exposed to vehicle or 10 nmol/l insulin for 5 min. Western blotting with anti-IRS-1, phospho-Akt or Akt antibody was performed. (b) VSMCs transfected with IRS-1 siRNA or scrambled siRNA were exposed to vehicle or 10 nmol/l insulin for 24 h. Protein synthesis was measured by [3H]-leucine incorporation. Data represent mean ± s.e. (n = 6), expressed as fold change compared with unstimulated cells. *P < 0.05 vs. control VSMCs. IRS-1, insulin receptor substrate-1; siRNA, small interfering RNA; VSMCs, vascular smooth muscle cells.