Table 1.

Behavioral characterization of TK-GFP and wild-type adult females

Behavior	WT, mean ± SEM (n = 10)	TK-GFP, mean ± SEM (n = 8)	p value
Time in the center (s), open field test	11.2 ± 1.1	9.0 ± 1.3	0.20
Distance traveled (cm), open field test	2030 ± 88	1978 ± 116	0.72
Time in the illuminated area (s), dark–light test	172 ± 38	176 ± 42	0.95
Number of transitions, dark–light test	37.2 ± 7.9	38.7 ± 8.0	0.90
Preference index to novel object (%), object-recognition task	59.1 ± 1.9	62.9 ± 3.2	0.30
Time exploring object mouse (s), social affiliation test	77.9 ± 5.1	84.2 ± 6.0	0.43
Number of escapes, social affiliation test	0.8 ± 0.25	1.37 ± 0.42	0.23

Wild-type (n = 10) and TK-GFP (n = 8) littermates (n = 10) were singly housed from PND 30–65 and tested in the following behaviors from PND 66–72: open-field, dark–light, object-recognition, and social affiliation test. These mice did not undergo intracerebroventricular surgery or exposure to GCV. However, exposure to intracerebroventricularly delivered PBS during this same period did not modify social behavior in TK-GFP mice (Fig. 7A,B), indicating that exposure to intracerebroventricularly delivered GCV is necessary to induce social abnormalities in TK-GFP female mice.