Table. Small mammals trapped during 2 subsequent winters (2008 and 2009) in TBEV-Eur– and TBEV-Sib–endemic areas, Finland*.

Location (virus subtype), year, and mammal species	No. animals trapped	No. RNA positive by real-time PCR			No. (%) antibody positive†
		Brain	Organ pool/spleen‡	Total (%)
Kokkola (TBEV-Sib)
2008
Myodes glareolus vole	63	1§	0	1 (1.6)	2 (3.2)
Sorex caecutiens shrew	4	0	0	0	0
S. araneus shrew	7	0	0	0	0
2009
M. glareolus vole	17	4§	2	5 (29.4)	4 (23.5)
S. araneus shrew	3	0	1	1 (33.3)	0
Isosaari (TBEV-Eur)
2008
Microtus agrestis vole	71	3¶	2	4 (5.6)	0
S. araneus shrew	7	0	0	0	0
2009
M. agrestis vole	24	13	0	13 (54.2)	2 (8.3)
S. araneus shrew	6	0	0	0	0

*TBEV, tick-borne encephalitis virus; TBEV-Eur, European subtype of TBEV; TVEV-Sib, Siberian subtype of TBEV.
†Blood samples, diluted ≈1:10 in phosphate-buffered saline, were screened by immunofluorescence assay with TBEV-infected Vero E6 cells as antigen.
‡For animals collected in 2008, organ pool of lungs, spleen, and liver were screened; for animals collected in 2009, only spleen was screened.
§Three brain samples positive for TBEV RNA by real-time reverse transcription–PCR (RT-PCR) (1 in 2008 and 2 in 2009) were also positive for the TBEV nonstructural protein (NS) 5 gene by nested RT-PCR.
¶Two brain samples positive for TBEV RNA by RT-PCR were also positive for the TBEV NS5 gene by nested RT-PCR.