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. Author manuscript; available in PMC: 2011 Oct 31.
Published in final edited form as: J Proteome Res. 2008 Oct 8;7(11):4670–4677. doi: 10.1021/pr800215n

Figure 1. Schematic illustrating the workflow of 18O labeling and comparison of membrane proteins derived from biliary tract and cholangiocarcinoma.

Figure 1

Membrane proteins were enriched from normal human biliary tract and cholangiocarcinoma, resolved by SDS-PAGE and subjected to trypsin digestion. The peptides derived from normal and cholangiocarcinoma membrane preparations were differentially labeled using 16O and 18O, respectively, and analyzed by liquid chromatography tandem mass spectrometry. Relative quantitation was carried out to determine the differential expression of the identified proteins.