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. Author manuscript; available in PMC: 2011 Oct 31.
Published in final edited form as: J Proteome Res. 2008 Oct 8;7(11):4670–4677. doi: 10.1021/pr800215n

Figure 3. Validation of candidate biomarkers by immunoblotting.

Figure 3

The membrane samples from normal bile duct and cholangiocarcinoma preparation were resolved by SDS-PAGE gels and blotted onto a nitrocellulose membrane. Detection of the target proteins was carried out by horseradish peroxidase conjugated system using enhanced chemiluminescence detection reagents.