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. Author manuscript; available in PMC: 2011 Oct 31.
Published in final edited form as: J Microsc. 2010 Sep 1;239(3):223–232. doi: 10.1111/j.1365-2818.2010.03376.x

Fig. 1.

Fig. 1

(a) Bright field TEM image (Iunfilt) of 100 nm unstained section of Epon embedded mouse thymocyte nucleus. Dark regions correspond to densely packed chromatin, rich in phosphorus and nitrogen. (b) Experimental electron energy loss spectrum (EELS) obtained from an area outlined in (a). The background-subtracted spectrum indicates the presence of phosphorus (P), nitrogen (N) and oxygen (O) in this specimen. The energy of P L2,3 pre-edge window used for background extrapolation is 120 ± 10 eV, and the P L2,3 post-edge window is at 152 ± 10 eV. Similarly, the N K pre-edge and post-edge windows are 375 ± 10 eV and 410 ± 10 eV, respectively.