Table 2.
Characteristics of mAb 37.1 and mAb 37surr
| Function | Cell type (human/cynomolgus) | mAb 37.1 | mAb 37surr |
|---|---|---|---|
| Apparent affinity, KD | B cells/B cells | 0.4nM | 0.2nM |
| T cells/T cells | 1.7nM | 1.4nM | |
| Ag density, sites/cell | B cells/B cells | 53 000 | 55 000 |
| T cells/T cells | 1900 | 1900 | |
| ADCC (EC50) | Ramos/macCD37-CHO | 0.01nM (2 ng/mL) | 0.02nM (4 ng/mL) |
| Apoptosis, without x-link (EC50) | Ramos/macCD37-CHO | 0.4nM (66 ng/mL) | 9.3nM (1423 ng/mL) |
| Apoptosis, with x-link (EC50) | Ramos/macCD37-CHO | 0.3nM (41 ng/mL) | 10nM (1574 ng/mL) |
| CDC | Ramos/macCD37-CHO | Negative | Negative |
| Whole blood (EC50) | B cells/B cells | 0.2nM (33 ng/mL) | 0.9nM (140 ng/mL) |
ADCC indicates Ab-dependent cell-mediated cytotoxicity; and CDC, complement-dependent cytotoxicity.