Table 2. Virus antigen expression and severity of lesion in different tissues of the lung of 3 ferrets inoculated with pandemic (H1N1) 2009 or reassortant pandemic (H1N1) 2009 viruses*.
| Virus |
Cumulative score per tissue |
||||||||||
| Bronchial surface epithelium |
|
Bronchial submucosal epithelium |
|
Bronchiolar epithelium |
|
Alveolar epithelium |
|||||
| IHC |
H&E |
IHC |
H&E |
IHC |
H&E |
IHC |
H&E |
||||
| Pandemic (H1N1) 2009 | 0, 0, 0† | 0, 0, 0 | 0, 0, 1 | 0, 0, 1 | 0, 0, 1 | 0, 0, 1 | 0, 0, 0 | 0, 0, 0 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H1N1) PB2PA | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H1N1) PB2 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H3N2) PB1NA | 1, 1, 0 | 0, 1, 0 | 2, 1, 0 | 1, 1, 0 | 2, 1, 0 | 1, 1, 0 | 1, 1, 0 | 1, 1, 1 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H3N2) NA | 1, 1, 1 | 0, 1, 1 | 1, 0, 3 | 1, 0, 3 | 2, 2, 3 | 2, 2, 3 | 1, 0, 1 | 1, 1, 1 | |||
*IHC, immunohistochemistry to detected virus antigen expression; H&E, hematoxylin and eosin staining to analyze severity of inflammation; PB, basic polymerase; PA, acidic polymerase; NA, neuraminidase. †Individual scores (as indicated in the Methods section) for 3 ferrets are listed.