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. 2011 Oct 10;121(11):4526–4536. doi: 10.1172/JCI45008

Figure 5. APOBEC3G enhances colon cancer cell migration and invasion through restoration of MMP2 from the inhibition of miR-29.

Figure 5

(A) Sequence alignment for miRNA-29 and its mRNA target MMP2 using TargetScan software. Square shows miR-29 consensus target sequence (left). Luciferase activity of pMIR-Report construct containing 3′ UTR of MMP2 miR-29 binding site was measured in 293 cells overexpressing miR-29 (right). *P < 0.05. (B) miR-29 and MMP2 mRNA levels in SW620 cells expressing miR-29 antagomir (si-miR-29) and L-1 cells expressing miR-29 precursor using quantitative PCR. (C) miR-29 and MMP2 mRNA levels in SW620 cells, L-1 cells expressing retroviral APOBEC-shRNA (si-APO) and si-miR-29 using quantitative PCR. (D) Immunoblotting was performed in L-1 cells knocking down APOBEC3G or miR-29 to examine mRNA and protein expression of the indicated genes. Wound healing assay was performed in (E) SW620 cells and (G) L-1 cells expressing the indicated constructs or oligonucleotides to measure cell migrating distance for 48 hours. The number of invasive cells was determined in (F) SW620 cells and (H) L-1 cells expressing the indicated constructs or oligonucleotides. Bars show the mean value of the representative results from 3 experiments, each conducted in duplicate (± SD).