Figure 3. The defect in Th17 cell generation in NOD.Il21r KO mice is both T cell intrinsic and APC driven.

(A) Naive T cells from NOD or NOD.Il21r KO mice were activated with plate-bound anti-CD3, and (B and C) naive T cells from NOD mice were activated with soluble anti-CD3 in the presence APCs, (A and B) in the presence or (C) absence of IL-6 and TGF-β. Intracellular staining was performed after 3 days. Values in each quadrant or gate represent the percentage of 7-AAD^–CD4⁺ cells. (C) Cell-conditioned media were assessed for IFN-γ production after 48 hours. Data are representative of 2 to 3 independent experiments.