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. 2011 Oct 3;121(11):4503–4515. doi: 10.1172/JCI57456

Figure 4. Cytokine regulation of GATA3 expression by Tregs.

Figure 4

(A) GATA3 expression in SILp Tregs is independent of STAT6 signaling. Cells were isolated from the SILp of naive BALB/c or STAT6-deficient mice and stained for expression of Foxp3 and GATA3. Plots are gated on live CD4+TCR-β+ cells. Numbers in quadrants refer to the percentage of each subset. (B) IL-6 and IL-27 impair expression of GATA3 by Tregs in vitro. CD4+eGFP+ T cells were cultured with SpDCs in the presence of anti-CD3 antibody and rIL-2 for 5 days in the presence of indicated cytokines. GATA3 expression was normalized to 100% of the IL-2 alone conditions. (C) STAT3 and T-bet negatively regulate GATA3 expression in SILp Tregs. Cells were isolated from the SILp of littermate control or Stat3f/f-CD4Cre (STAT3 KO) or Tbx21-deficient mice and stained for expression of Foxp3 and GATA3 intracellularly. FACS plots of control are gated on live CD4+TCRβ+ cells. n = 3 mice per group. **P < 0.008; ***P ≤ 0.0003. (D) Graphical representation of FACS plots in C. Data shown are representative of 2 (A, C, and D) and 3 (B) independent experiments with similar results. Data are presented as mean ± SEM.