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. 2011 Oct 10;121(11):4281–4288. doi: 10.1172/JCI58554

Figure 1. Skeletal muscle Sirt1 deacetylase activity modulates enhanced in vivo insulin action after CR.

Figure 1

(A) Ac-p53 in skeletal muscle. p53 was immunoprecipitated from whole cell lysate (WCL) with anti-p53, and immunoprecipitates were immunoblotted with anti–acetyl lysine (Ac-Lys) and anti-p53. (B) Ac-Pgc1α in skeletal muscle. Acetylated proteins were immunoprecipitated from whole cell lysate with anti–acetyl lysine and immunoblotted for Pgc1α. Pgc1α was also measured in the whole cell lysate used for immunoprecipitation. (C) Blood glucose concentration and (D) area under the blood glucose curve (AUC) during an OGTT (1 g/kg). n = 8–10/group. (E) GINF, (F) IS-GDR, and (G) percentage suppression of HGP during a hyperinsulinemic-euglycemic clamp. n = 6–8/group. A 2-way ANOVA for main effects of diet and genotype was used for statistical analysis, with Tukey post-hoc test. *P < 0.05, within same genotype; #P < 0.05, within same diet group. Data are mean ± SEM.