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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Struct Biol. 2010 Dec 4;174(1):203–212. doi: 10.1016/j.jsb.2010.11.027

Figure 2.

Figure 2

TEM-images of amelogenin rH174 at different times of incubation; top row (a–c) rH174 was dissolved in aqueous suspensions at pH 2–3, subsequently pH was raised 4.5 using KOH and Tris-buffer. The characteristic 20 nm nanosphere of amelogenin was observed at all time points between 1 to 14 days of incubation, t = 24 h (a); occasionally nanosphere aggregated into short strings, t = 48 h (b), and t = 7 days (c). When aqueous amelogenin suspensions were mixed at pH 2–3 with a non-polar solvent (octanol/ethyl acetate) and subsequently pH was raised to 4.5, ribbon-like nano-structures appeared as early as 24 hours (d). Ribbons grew in length over time and arranged themselves in a parallel manner, t = 7 days (e); ribbons reached several micrometer in length and formed well aligned bundles of nanoribbons, t= 14 days (f).