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TEM analysis of amelogenin nanoribbons showed the presence of a dark central line which was present in the absence of stain and when specimens were positively or negatively stained with nano-W (a). Averaging over 604 equally sized ribbon segments (negative stain) further revealed the evidence of a dark central line that indicated higher electron density which could be associated with the presence of calcium and/or phosphate ions which were both required for self-assembly into ribbons.
