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. Author manuscript; available in PMC: 2011 Oct 31.
Published in final edited form as: Nature. 2009 Mar 22;459(7243):98–102. doi: 10.1038/nature07883

Figure 3. Zebrafish fu has a Hh-independent role in L-R asymmetry and generation of 9+2 cilia.

Figure 3

a, b, Section in situ hybridization to Fu (pink signal) in chick trachea (a) and X. tropicalis testis (b). Arrow indicates sites of Fu expression. c, Whole mount in situ hybridization to cmlc2 (purple signal) in smohi1640Tg fish embryos at 24 hpf. View is dorsal. d, e, Whole-mount in situ hybridization to cmlc2 in wt (d) and fu morphants at 24 hpf. View is dorsal. (e). f, Summary of cardiac laterality defects in wt (n = 206), fu morphants (n = 130), and fu morphants rescued with mouse Fu (n = 134). L, left; R, right; M, medial. g, h, Whole mount in situ hybridization to spaw at 15-somite stage. View is dorsal. i, Summary of spaw expression in the lateral plate mesoderm (LPM) in wt (n = 116), fu morphants (n = 92), and fu morphants rescued with mouse Fu (n = 73). L, left; R, right; A, absent; B, bilateral. j, k, Electron micrograph of Kupffer’s vesicle (KV) cilia from wt (j) and fu morphant (k). l, Quantification of ultrastructural defects in KV cilia from wt and fu morphants. Error bars indicate standard deviation (s.d.). m, n, Whole mount in situ hybridization to cmlc2 in wt and kif7 morphants at 24 hpf. View is dorsal. o, Summary of cardiac laterality defects in wt (n = 87) and kif7 morphants (n = 109). p, Summary of essential Fu and Cos2/Kif27 functions in metazoan model organisms.