Figure 1.
The deactivation time course for Gly and GABA receptors is shaped by the intracellular chloride concentration, regardless of the direction of current flow. Outside-out current responses elicited by 1 ms, 3 mm Gly or GABA decay more quickly if intracellular chloride is low (i.e., 10 mm, gray traces in b, pipette solution internalCl-10; Table 1) than in the presence of high intracellular chloride (131 mm, black traces, pipette solution internalCl-131; Table 1; in all recordings in b, extracellular chloride was 131 mm, solution externalCl-131). The current is inward (chloride goes out) when the patch is held at −100 mV and outward at +20 mV. a, Summary of the weighted τ of the current relaxations. b, Representative outward and inward currents in the presence of 10 mm (gray trace) or 131 mm intracellular Cl (black trace). Traces are averages of 5–10 individual responses, normalized to the peak to allow comparison of their decay phase. Time constant values are reported in Table 2.