Table 1. Primers used for PCR and sequencing analysis of genes, involved in the LPS synthesis, examined in this study.
PCR | Primers | Sequence (5′→3′) | Specificity (gene) | Locus* | Putative role** | Amplicon size (bp) |
347 | 1153 | AGTGGACGAAACTTGGGATG | wboB | BMEI 0997 | Mannosyltransferase | 1686 |
1154 | ATCTCTGCATTCGTGGGAAG | |||||
348 | 1156 | GGGTTATAGCCGATAAACACG | wboA | BMEI 0998 | Glycosyltranferase | 1297 (2139 in RB51) |
1157 | CCCGCGCATTAAGAGTAGAC | |||||
349 | 1159 | ATGAGCGGACCATAAAGGTG | wa 1326 | BMEI 1326 | protein of unknown function | 2253 |
1160 | GCGCTGATTGAGGAAAAGAC | |||||
350 | 1162 | ATGCCTCATCTACGCTCCAT | wbkE | BMEI 1393 | Glycosyltranferase | 1287 |
1163 | TGTCGAATTTTTGGTTGTCG | |||||
351 | 1165 | CGACAACCAAAAATTCGACA | manAOAg | BMEI 1394 | Perosamine synthesis | 1256 |
1166 | GAGGATGATTTCGGACGTGT | |||||
352 | 1168 | GACAACCAAAGAGGCAAAGC | manBOAg | BMEI 1395 | Perosamine synthesis | 1592 |
1169 | TGCAAAGCTCTCTGAAACGA | |||||
353 | 1171 | GCGGATGAATTCAGATCGTT | manCOAg | BMEI 1396 | Perosamine synthesis | 1466 |
1172 | GTGCCATCCATCATGTGAAC | |||||
354 | 1174 | CCTCCTTGCTATCCGATTGA | wbkA | BMEI 1404 | Glycosyltranferase | 1271 |
1175 | ATTTGCAAAATCTGGGCAAC | |||||
355 | 1177 | TGTAATGCTCCACGAGCAAG | gmd | BMEI 1413 | Perosamine synthesis | 1257 |
1178 | ACCCTGTATCAAGGCATTCG | |||||
356 | 1180 | GCTGGAAGCCGAAAATCTCT | per | BMEI 1414 | Perosamine synthesis | 1297 |
1181 | CACCAGAAGTGGCGTACCTT | |||||
357 | 1183 | AGCGCTTCCACAGGATCTTA | wzm | BMEI 1415 | ABC transporter permease | 995 |
1184 | ATGCAGCGTAGTGCAGATGA | |||||
358 | 1186 | TGCTTAGGTGTGAGCCTCCT | wzt | BMEI 1416 | ABC transporter permease | 915 |
1187 | AGAGGGACCTCACATTCTCG | |||||
359 | 1189 | AACTCGGGAATGGGAGCTAT | wbkB | BMEI 1417 | Unknown | 934 |
1190 | CAAAACGCGTGTATTTGGTG | |||||
360 | 1192 | CGTTACCCAAGTTGGAATGC | wbkC | BMEI 1418 | Formyltranferase | 890 |
1193 | CTGCTGGGACCAAAAACCTA | |||||
361 | 1195 | GTTAGCGGAGGAATGGACAA | wbkF (ex wecA) | BMEI 1426 | Undecaprenyl-P-α-N-acetylglucosaminyl | 1213 |
1196 | CGCCATGGAAAAGAAGGATA | |||||
362 | 1198 | CCACACTCATTCTGCTGCTC | wbkD | BMEI 1427 | Unknown | 2042 |
1199 | GGTCTGTTACGGTTGCTGGT | |||||
363 | 1201 | CACTTGCTGCCTTGATTGAG | pgm | BMEI 1886 | Phosphoglucomutase | 1956 |
1202 | TCACTGCCCTACTGCCCTAC | |||||
364 | 1204 | ACAGGAGAATTTTCGGCGTA | wa 0053 | BMEII 0053 | Transport ATPase | 976 |
1205 | ATCGCTGTTGCAACCTACCT | |||||
366 | 1210 | GGCAGATACAGGTTCGATGG | manB-core | BMEII 0899 | Phosphomannomutase | 1577 |
1211 | GGTCTTGCCGATGAGTGTTT | |||||
367 | 1213 | TGGAATCTTCGGATGACACA | manC-core | BMEII 0900 | Unknown | 1526 |
1214 | TTCGCATTTGGCTGAATATG | |||||
368 | 1216 | GATGTGGGACTTCCGTTCTG | wa**** | BMEII 1134 | Amidase (?) | 1290 |