Skip to main content
. 2011 Oct 31;6(10):e24073. doi: 10.1371/journal.pone.0024073

Table 1. Primers used for PCR and sequencing analysis of genes, involved in the LPS synthesis, examined in this study.

PCR Primers Sequence (5′→3′) Specificity (gene) Locus* Putative role** Amplicon size (bp)
347 1153 AGTGGACGAAACTTGGGATG wboB BMEI 0997 Mannosyltransferase 1686
1154 ATCTCTGCATTCGTGGGAAG
348 1156 GGGTTATAGCCGATAAACACG wboA BMEI 0998 Glycosyltranferase 1297 (2139 in RB51)
1157 CCCGCGCATTAAGAGTAGAC
349 1159 ATGAGCGGACCATAAAGGTG wa 1326 BMEI 1326 protein of unknown function 2253
1160 GCGCTGATTGAGGAAAAGAC
350 1162 ATGCCTCATCTACGCTCCAT wbkE BMEI 1393 Glycosyltranferase 1287
1163 TGTCGAATTTTTGGTTGTCG
351 1165 CGACAACCAAAAATTCGACA manAOAg BMEI 1394 Perosamine synthesis 1256
1166 GAGGATGATTTCGGACGTGT
352 1168 GACAACCAAAGAGGCAAAGC manBOAg BMEI 1395 Perosamine synthesis 1592
1169 TGCAAAGCTCTCTGAAACGA
353 1171 GCGGATGAATTCAGATCGTT manCOAg BMEI 1396 Perosamine synthesis 1466
1172 GTGCCATCCATCATGTGAAC
354 1174 CCTCCTTGCTATCCGATTGA wbkA BMEI 1404 Glycosyltranferase 1271
1175 ATTTGCAAAATCTGGGCAAC
355 1177 TGTAATGCTCCACGAGCAAG gmd BMEI 1413 Perosamine synthesis 1257
1178 ACCCTGTATCAAGGCATTCG
356 1180 GCTGGAAGCCGAAAATCTCT per BMEI 1414 Perosamine synthesis 1297
1181 CACCAGAAGTGGCGTACCTT
357 1183 AGCGCTTCCACAGGATCTTA wzm BMEI 1415 ABC transporter permease 995
1184 ATGCAGCGTAGTGCAGATGA
358 1186 TGCTTAGGTGTGAGCCTCCT wzt BMEI 1416 ABC transporter permease 915
1187 AGAGGGACCTCACATTCTCG
359 1189 AACTCGGGAATGGGAGCTAT wbkB BMEI 1417 Unknown 934
1190 CAAAACGCGTGTATTTGGTG
360 1192 CGTTACCCAAGTTGGAATGC wbkC BMEI 1418 Formyltranferase 890
1193 CTGCTGGGACCAAAAACCTA
361 1195 GTTAGCGGAGGAATGGACAA wbkF (ex wecA) BMEI 1426 Undecaprenyl-P-α-N-acetylglucosaminyl 1213
1196 CGCCATGGAAAAGAAGGATA
362 1198 CCACACTCATTCTGCTGCTC wbkD BMEI 1427 Unknown 2042
1199 GGTCTGTTACGGTTGCTGGT
363 1201 CACTTGCTGCCTTGATTGAG pgm BMEI 1886 Phosphoglucomutase 1956
1202 TCACTGCCCTACTGCCCTAC
364 1204 ACAGGAGAATTTTCGGCGTA wa 0053 BMEII 0053 Transport ATPase 976
1205 ATCGCTGTTGCAACCTACCT
366 1210 GGCAGATACAGGTTCGATGG manB-core BMEII 0899 Phosphomannomutase 1577
1211 GGTCTTGCCGATGAGTGTTT
367 1213 TGGAATCTTCGGATGACACA manC-core BMEII 0900 Unknown 1526
1214 TTCGCATTTGGCTGAATATG
368 1216 GATGTGGGACTTCCGTTCTG wa**** BMEII 1134 Amidase (?) 1290

GeneBank AE008917 (chromosome I) and AE008918 (chromosome II);

**Moriyon et al. (2004).