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. 2011 Oct 31;6(10):e26839. doi: 10.1371/journal.pone.0026839

Figure 4. Expression of MUC16 in a panel of PC cell lines using western blot, RT-PCR and confocal studies.

Figure 4

(A) Western blot analysis of MUC16 expression in PC cell lines. Protein lysates from eighteen PC cell lines were resolved on a 2% SDS-agarose gel. MUC16 expression was observed in DanG, HPAC, SU86.86, Colo357, CD18/HPAF, Capan1 and T3M4 cell lines. β-actin was used as an internal control (B) RT-PCR analysis of MUC16 expression in various PC cell lines. Actin was used as an internal control. (C) Immunofluorescence studies of two cell lines (CD18 and Capan1) that expresses MUC16 and two cell lines (CD11 and SUIT2) that do not express MUC16.